abstract：:Streptococcus bovis is commonly present in the rumen, but strains of S. bovis have also occasionally been isolated from human blood or fecal samples. Studies were undertaken with 16s rRNA gene sequences and DNA hybridizations to define the genetic relationships between these two groups of strains. Ruminal strains were...

journal_title：Current microbiology

authors： Nelms LF,Odelson DA,Whitehead TR,Hespell RB

更新日期：1995-11-01 00:00:00

abstract：:NADPH-dependent aldose reductase activity induced by D-xylose or L-arabinose was detected in cell-free extracts of Candida guilliermondii, but only negligible activities were observed if D-glucose served as carbon source. The induction of aldose reductase activity on mixed sugars was investigated under resting cell co...

journal_title：Current microbiology

authors： Sugai JK,Delgenes JP

更新日期：1995-10-01 00:00:00

abstract：:Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observe...

journal_title：Current microbiology

authors： Novotná J,Li XM,Novotná J,Vohradský J,Weiser J

更新日期：1995-08-01 00:00:00

abstract：:The cbbF genes of the facultative chemoautotroph Alcaligenes eutrophus H16 are part of two highly homologous cbb operons. Both the chromosomal and the megaplasmid pHG1-borne copy of cbbF were cloned and sequenced. Subsequent analyses including comparison with known sequences from other organisms and heterologous expre...

journal_title：Current microbiology

authors： Yoo JG,Bowien B

更新日期：1995-07-01 00:00:00

abstract：:Nine phage isolates infectious for Vibrio vulnificus and falling into four morphological groups were isolated from estuarine waters collected in Louisiana. Of the 60 V. vulnificus strains tested, 87% were susceptible to one or more of the isolates. With the exception of V. fluvialis, Vibrio species other than vulnific...

journal_title：Current microbiology

authors： Pelon W,Siebeling RJ,Simonson J,Luftig RB

更新日期：1995-06-01 00:00:00

abstract：:A 4.5-kilobase DNA fragment from Buchnera aphidicola, the endosymbiont of the aphid Schizaphis graminum, was cloned and sequenced. On the basis of homology to Escherichia coli, the following genes were found in the order listed: aroH-thrS-infC-rpmI-rplT. AroH corresponds to the E. coli tryptophan-inhibited 3-deoxy-D-a...

journal_title：Current microbiology

authors： Kolibachuk D,Rouhbakhsh D,Baumann P

更新日期：1995-05-01 00:00:00

abstract：:Legionella and amebae populations in 16 cooling towers were challenged with three commercially available biocide formulations. The active agents were: a chlorinated phenolic thioether (CPTE), bromo-nitro-propane-diol (BNPD), and bromo-chloro-dimethylhydantoin (BCD, in briquette form). The towers were dosed with these ...

journal_title：Current microbiology

authors： Bentham RH,Broadbent CR

更新日期：1995-03-01 00:00:00

abstract：:Rep22 is the replication region of the lactococcal theta replicating pUCL22 plasmid. The copy number of Rep22-based plasmids in Lactococcus was determined by using a chromosomal DNA fragment from Lactococcus lactis subsp. lactis MMS368 as reference. Segregational behavior appeared to be linked to copy number and there...

journal_title：Current microbiology

authors： Frère J,Herreman C,Boutibonnes P,Novel M,Novel G

更新日期：1995-01-01 00:00:00

abstract：:A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based primers by use of the polymerase chain reaction, and then digested with th...

journal_title：Current microbiology

authors： Porteous LA,Armstrong JL,Seidler RJ,Watrud LS

更新日期：1994-11-01 00:00:00

abstract：:A Chinese HCV cDNA clone, designated as Q379, 379 nucleotides in length, was selected by recombinant immunoscreening from a random-primed Chinese HCV lambda gt11 library. DNA sequencing demonstrated that Q379 situated from positions 7314 to 7695 at NS5 region corresponded with the American prototype HCV nucleotide seq...

journal_title：Current microbiology

authors： Qi Z,Arima T

更新日期：1994-03-01 00:00:00

abstract：:The neurotoxin gene of non-proteolytic Clostridium botulinum type B (strain Eklund 17B) was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated with primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 3' end of the gene was obtained by using primer...

journal_title：Current microbiology

authors： Hutson RA,Collins MD,East AK,Thompson DE

更新日期：1994-02-01 00:00:00

abstract：:The limitation of phosphate concentrations in effluents is of international concern because of the risk of eutrophication.Acinetobacter spp. have often been isolated from activated sludge plants exhibiting enhanced phosphate removal. The ability to remove phosphate from mixed liquor byAcinetobacter isolates and refere...

journal_title：Current microbiology

authors： Bosch M,Cloete TE

更新日期：1993-09-01 00:00:00

abstract：:The DNA sequence was determined for a region upstream of the puf operon of Rhodospirillum rubrum. A partial ORF was identified. The DNA sequence and the inferred amino acid sequences were aligned with those of bchA of Rhodobacter capsulatus and other phototrophic bacteria. Based on this alignment and genetic evidence,...

journal_title：Current microbiology

authors： Lee IY,Collins ML

更新日期：1993-08-01 00:00:00

abstract：:Several di-, tri-, and oligopeptides were incubated individually in vitro with rumen fluid from two sheep receiving a mixed grass hay/concentrate diet and with washed cells of Prevotella (formerly Bacteroides) ruminicola M384 and P. ruminicola B(1)4. The rates of breakdown of most peptides were similar in the rumen fl...

journal_title：Current microbiology

authors： Wallace RJ,McKain N,Broderick GA

更新日期：1993-06-01 00:00:00

abstract：:The cyclomaltodextrinase gene from Bacillus subtilis high-temperature growth transformant H-17 was cloned on separate PstI, BamHI, and EcoRI fragments into the plasmid vector pUC18, but was expressed in an inactive form in the host, Escherichia coli DH5 alpha. High level constitutive expression of the gene product was...

journal_title：Current microbiology

authors： Krohn BM,Lindsay JA

更新日期：1993-04-01 00:00:00

abstract：:An alkaline phosphatase (phoA) gene from Zymomonas mobilis was isolated in Escherichia coli CC118 by use of the plasmid Bluescript KS+. The origin of the 6.4-kb DNA fragment in pZAP1 from the chromosome of Z. mobilis was confirmed by Southern blotting and hybridization studies. The Z. mobilis phoA gene was localized a...

journal_title：Current microbiology

authors： Karunakaran T,Gunasekaran P

更新日期：1992-11-01 00:00:00

abstract：:Polymorphisms in enzymatically amplified ribosomal DNA (rDNA) were examined in 18 strains of Saccharomyces. Restriction patterns generated from the region spanning the internal transcribed spacers (ITS) and the 5.8S rDNA produced two clusters corresponding to S. bayanus and S. cerevisiae. The type culture of S. carlsb...

journal_title：Current microbiology

authors： Molina FI,Inoue T,Jong SC

更新日期：1992-11-01 00:00:00

abstract：:Strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice ve...

journal_title：Current microbiology

authors： Biavati B,Crociani F,Mattarelli P,Scardovi V

更新日期：1992-07-01 00:00:00

abstract：:Glutamine synthetase (GS) fromBeneckea alginolytica strain 90 was purified to homogeneity as indicated by sodium dodecyl sulface polyacrylamide gel electrophoresis. The purified enzyme was injected into rabbits, and the antiserum, as well as previously prepared antiserum against the GS fromEscherichia coli, was used i...

journal_title：Current microbiology

authors： Baumann L,Baumann P

更新日期：1980-07-01 00:00:00

abstract：:Corrinoids in several diverse species of methanogens were quantified by a bioassay utilizingEscherichia coli 113-3, a corrinoid auxotroph. All five species examined contained >0.65 nmol corrinoid/mg dry cells when grown on H2/CO2 as carbon and energy source. The highest corrinoid levels (4.1 nmol/mg cells) were found ...

journal_title：Current microbiology

authors： Krzycki J,Zeikus JG

更新日期：1980-07-01 00:00:00

abstract：:The process ofBeggiatoa trichome division was elucidated through phase-contrast microscopy and transmission and scanning electron microscopy. Trichome breakage and dispersion is accomplished by the formation of sacrificial cells (necridia) at various points within the trichome. Upon dying, the sacrificial cells lyse, ...

journal_title：Current microbiology

authors： Strohl WR,Larkin JM

更新日期：1978-01-01 00:00:00